mouse igg2a anti myosin 7a myo7a (Santa Cruz Biotechnology)
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Mouse Igg2a Anti Myosin 7a Myo7a, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 85 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 85 article reviews
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1) Product Images from "Fatty acid binding protein type 7 deficiency preserves auditory function in noise-exposed mice."
Article Title: Fatty acid binding protein type 7 deficiency preserves auditory function in noise-exposed mice.
Journal: Scientific reports
doi: 10.1038/s41598-023-48702-4
Figure Legend Snippet: Figure 1. Experimental design, body weights, and FABP7 expression in the cochlea. Schematic of the experiments using wild-type (WT) and Fabp7 knockout (KO) mice (A). The body weight (BW) of Fabp7 KO mice was not significantly different from that of WT mice (B). Immunohistochemistry of FABP7 in the cochlea of WT mice revealed that FABP7 was expressed throughout the spiral ganglion (SG), organ of Corti (OC), spiral limbus (SLim), and spiral ligament (SLig) (C). Scale bar, 200 μm (C–D). No expression of FABP7 was observed in the cochlea of Fabp7 KO mice (D). Maximum projection images of the OC showing high expression of FABP7 in Schwann cells and SOX2-positive supporting cells around the MYO7a-positive outer hair cells (OHCs) in the cochlea of WT mice (E). In the SG, FABP7 was expressed in satellite cells surrounding TUJ-1-positive neurons (F). Scale bars, 50 μm (E–F). Auditory brainstem response; ABR, weeks; W, post-noise exposure day; PNED, inner hair cell; IHC. Statistical significance was determined using two-way analysis of variance, followed by Šídák multiple comparison test. Error bars represent standard deviation.
Techniques Used: Expressing, Knock-Out, Immunohistochemistry, Comparison, Standard Deviation
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Incubation:Article Title: Fatty acid binding protein type 7 deficiency preserves auditory function in noise-exposed mice. Article Snippet: Sections were mounted on MAS-coated glass slides (Superfrost; Matsunami Glass, Kishiwada, Japan). .. After rinsing with PBS, tissue sections were blocked with 3% bovine serum albumin/0.3% Triton X-100/ PBS for 30 min at room temperature, the unconjugated AffiniPure Fab fragment of anti-mouse IgG (1:10, Jackson ImmunoResearch, West Grove, PA) for 2 h at room temperature, and incubated overnight with the following primary antibodies: (1) rabbit IgG anti-FABP7 (1:1000, Merck, Darmstadt, Germany #PRS4259), (2) mouse IgG anti-TUJ-1 (1:500, Abcam, Cambridge, UK #ab78078), (3) Article Title: Fatty acid binding protein type 7 deficiency preserves auditory function in noise-exposed mice Article Snippet: Sections were mounted on MAS-coated glass slides (Superfrost; Matsunami Glass, Kishiwada, Japan). .. After rinsing with PBS, tissue sections were blocked with 3% bovine serum albumin/0.3% Triton X-100/ PBS for 30 min at room temperature, the unconjugated AffiniPure Fab fragment of anti-mouse IgG (1:10, Jackson ImmunoResearch, West Grove, PA) for 2 h at room temperature, and incubated overnight with the following primary antibodies: (1) rabbit IgG anti-FABP7 (1:1000, Merck, Darmstadt, Germany #PRS4259), (2) mouse IgG anti-TUJ-1 (1:500, Abcam, Cambridge, UK #ab78078), (3) |